Support description: When new mechanisms of diseases are
investigated, it is important to have plasma or tissue levels
of certain endogenous molecules known to be the markers of the
diseases. These assays are often the working horses to help
investigators to build efficacy models and select drug
candidates. Examples of these molecules are dopamine,
homocysteine (HCY), S-Adenosyl-HCY and S-adenosyl-methionine,
small peptides, nucleosides, nucleotides, and small
oligonucleotides etc. We have expertise in this area to help
you get the valuable information.
Methods and procedure: Because these molecules are
endogenous, appropriate pseudo control matrices (e. g.
stripped plasma, PBS buffer etc) will be investigated to
construct calibration curves. Stable isotope labeled internal
standards will always be used. Parallelism between the pseudo
matrix and the actual one will be validated. Depending on the
chemical and physical properties of the target molecules,
suitable sample preparation methods such as solid phase
extraction will be applied before LC-MS/MS analysis. Because
these molecules are often related in part of biological
reactions, we will work with customers to seek appropriate
procedures such as conversion to ensure the stability of the
samples and during storage and sample handling. Depending on
the nature of the studies, appropriate depth of validation
will be adopted.
Quality control: QCs prepared in both pseudo and real
matrices will be measured. Precision (%CV) has to be within
20%. Accuracy has to be within ±25% of nominal values.
Data reporting: Concentrations of target markers in samples
dosed with vehicle, investigational drugs, or any diet
treatment will be reported in excel table format. All data
related to analytical performance (precision, accuracy of QCs
and standards, linear regression coefficient etc) will be
reported as part of the content.
Discovery
