Support description: Identification of metabolites from both in vitro test and in vivo animal studies are often necessary in the discovery and pre-clinic development of a drug candidate. In vitro experiments, using animal or human hepatocytes and microsomes, can provide a rapid and cost-effective screening to identify potential toxic metabolites.

Methods and procedure: After drug candidates (5 or 10 mM) reacting with liver microsomes and hepatocytes for a fixed period of time, major metabolites are profiled using MSⁿ technologies on triple quadruple and 3D Ion trap mass spectrometers.

Data reporting: Major metabolites based on the compound structure are reported.  We would also monitor and report other metabolite of interest upon customer's request.