Metabolic Stability in Liver Microsomes and
Hepatocytes
ProtTech Analytical provides service in testing stability of
drug candidates in liver microsome and hepatocyte in species
of rat, dog, monkey and human. Liver microsome (20 mg protein/mL)
and hepatocyte (1x106 cells/min) are freshly prepared before
each experiment. Default drug concentration of 0.5 or 1 µM is
used in the reaction. Positive control is used to ensure the
success of each reaction. Default sampling time points are at
0, 15, 30, 60 and 120 min or at any time points requested by
customers. Samples are analyzed by LC-MS/MS. Peak area ratios
of drug candidate over internal standard at all time points
are reported.
Discovery
